Researcher, CNRS and Paris Descartes University, France
In order to counter the effect of antibiotics, Gram-negative bacteria have developed an armoury of strategies among which active efflux of the drugs out of the bacteria plays a prominent role. The OprM/MexA/MexB tripartite efflux pump is responsible for the constitutive active efflux of various compounds in Pseudomonas aeruginosa. Our laboratory (Laboratory of Crystallography and NMR, UMR 8015 & Paris Descartes University) has developed a renowned technical know-how in the overexpression, solubilization, purification, and crystallization of each component of this pump. From a functional point of view, we are developing in vitro transport assays for the MexA – MexB – OprM efflux pump.