The Tucker Laboratory was established at the University of Strathclyde in 2009 with a key research focus on the molecular biology and antibiotic susceptibility of Pseudomonas aeruginosa. Our research ranges from protein structural dynamics all the way up to genomics and transcriptomics. We have a close collaboration with the Department of Pure and Applied Chemistry at Strathclyde and have established a range of antibiotic leads around the general theme DNA-binding molecules. Many of these molecules have strong activity against P. aeruginosa in the absence of an efflux pump inhibitor. We have an established high-throughput antibiotic screening method for both P. aeruginosa and Staphylococcus aureus. I have been responsible for establishing a next generation sequencing platform in my lab and we have used this for RNA-Seq analysis of antibiotic treated bacteria in order to study the mode of action of our lead compounds. We have also used genomics to compare clinical P. aeruginosa strains and to determine how this versatile pathogen adapts to the human niche during chronic lung infections and I am a founding member of the International Pseudomonas Genomics Consortium. My lab also hosts projects that investigate Streptomyces bacteria for novel antibiotic discovery, an interest that was fostered during my time as a Post-Doc at the BBSRC John Innes Centre.
Research in my laboratory is funded by the BBSRC and MRC.
You can follow my lab’s activities and progress on twitter @Tucker303 and on the web at http://spider.science.strath.ac.uk/sipbs/staff/Nicholas_Tucker.htm